Field & University Trial Data

Poultry Benefits

Ten real-world case studies — breeder farms, broiler operations, and university trials — showing AQUATIZE®'s measured impact on mortality, hatchability, feed conversion, and pathogen load.

Why It Matters

Bacterial and Fungal Diseases of Poultry⁷

Most of the pathogens responsible for major poultry diseases — in both the GI and respiratory tract — rely on nitrate reductase, the same enzyme AQUATIZE® exploits.

Site	Disease	Causative Organism	Nitrate Reductase
GI Tract	Colibacillosis (Coliform infection)	Escherichia coli	+
GI Tract	Necrotic Enteritis	Clostridium perfringens	+
GI Tract	Ulcerative Enteritis (Quail disease)	Clostridium colinum	+
GI Tract	Pullorum Disease	Salmonella pullorum	+
GI Tract	Fowl Typhoid	Salmonella gallinarum	+
GI Tract	Coccidiosis	Candida albicans	+
GI Tract	Fusariotoxicosis	Fusarium genus	+
Respiratory	Colibacillosis (Coliform infection)	Escherichia coli	+
Respiratory	Mycoplasmosis (CRD, Air Sac, Sinusitis)	M. gallisepticum, M. meleagridis, M. synoviae	?
Respiratory	Fowl Cholera	Pasteurella multocida	+
Respiratory	Infectious Coryza	Haemophilus gallinarum	+
Respiratory	Aspergillosis	Aspergillus fumigatus	+
Respiratory	Aflatoxicosis	Aspergillus and Penicillium genera	+

⁷ From The Poultry Site, Diseases of Poultry, Dr. Ivan Dinev.

Peer-Reviewed Foundations

Historical Scientific Results

• The fecal bacteria count just prior to processing chickens may be reduced with AQUATIZE (1:2000 dilution).⁸
• AQUATIZE reduced the effect of Salmonella enteritidis broiler infection, ameliorated the impact of Bordetella avium turkey infection, and increased nitrogen retention.⁹
• Both AQUATIZE and feed medication (bacitracin, 55 ppm) ameliorated the impact of Clostridium perfringens in broiler chickens reared to 21 days of age.¹⁰

⁸ Perdue and Jones, N.C. State, Poultry Science 72:253–266, '93.
⁹ Perdue and Luginbuhl, N.C. State, Avian Diseases 42:140–143, '96.
¹⁰ McNaughton and Keschen, Sol. BioSci, Inc., Poultry Science 79(Sup 1) p. 123.

Real-World Case Studies

Case 1 — Breeder Farm

2 houses, ≈9,000 hens/house, Cobb 500

Test Period	AQUATIZE	Mortality %	Total Eggs/HH*	Hatching Eggs/HH*	Hatch %	Kg Feed/Dozen Eggs	# Roosters Spiked**
2012–13 (25–62 wks)	No	18.3	150.0	144.7	85.4	3.173	1,000
2013–14 (26–65 wks)	Yes	13.2	176.3	171.8	87.5	2.727	530
Improvement		5.1	17.5%	18.7%	2.1%	14.0%	47%
Cobb Std. (26–65 wks)		7.74	179.9	175.3	86.1	—	—

* Hen Housed · ** The total cost of one rooster is about $14 to the company.

Weekly hatchability on the AQUATIZE®-treated flock tracked closely with the Cobb standard across the 26–66 week lay period — peaking in the low-to-mid 90% range mid-cycle before the expected decline toward the end of lay, mirroring the season-over-season improvement seen in the table above.

Case 2 — Broilers Grown on Built-Up Litter

0–42 days, ± AQUATIZE

~1.91

Untreated
Control

~1.90

Bleach

~1.87

AQ
1:5000

~1.85

AQ
1:3500

~1.83

AQ
1:2000

~1.82

AQ
1:1000

Feed Conversion (lower is better)

AQ = AQUATIZE. Values approximate, read from chart scale (1.78–1.92).

Case 3 — AQUATIZE Only in Last 24 Hours Before Catch & Haul

46-day-old broilers

• AQUATIZE at various dilutions, as well as bleach, were applied to broilers for 24 hours before harvest.
• Fecal bacterial counts were analyzed and results of percent reduction were reported.
• Birds treated with AQUATIZE at 1:2000 had the lowest fecal bacterial counts.

~5%

Control

~20%

Bleach

~48%

1:5000
AQ

~58%

1:3500
AQ

~65%

1:2000
AQ

Reduction in Fecal Bacteria (% killed)

Values approximate, read from chart scale (0–70%).

Case 4 — AQUATIZE and Necrotic Enteritis

Dual infection of Coccidia and Clostridia — test conducted by Dr. McNaughton at PARC Inst.

Trt Group	21-D Weight (g)	Feed Conv. (gr/gr)	Lesion Score	Mortality (%)	Fecal CFU (% reduced)
UC	520^a	1.358^a	0.14^a	3.0	11%
IC	383^d	1.414^c	2.64^c	8.0	—
IC+Ab	430^c	1.421^b	1.61^b	4.0	24%
IC+Aq	485^b	1.372^b	0.74^b	3.0	92%
IC+Aq+B	505^ab	1.364^ab	0.53^b	3.0	92%
IC+B	416^c	1.452^c	1.82^c	4.0	35%

Letter superscripts that differ are statistically significantly different at p<0.05. Intestinal lesions were scored as: 0 = no redness present; 1 = red spots and/or strikes present; 2 = ¼–½ of the intestine is red; 3 = intestine is completely red and covered.

Uninfected Control (UC) · Infected Control (IC) · IC + 66 ppm salinomycin and 55 ppm bacitracin (IC+Ab) · IC + AQUATIZE, 1:2,000 dilution into drinking water (IC+Aq) · IC + AQUATIZE + 0.30% NaHCO₃ (IC+Aq+B) · IC + 0.30% NaHCO₃ (IC+B)

Case 5 — Field Trial at a USA Poultry Company

Used at a dilution of 1:2000

Parameter	Control	AQUATIZE	Advantage
Chicks Placed	1 million	0.5 million	—
Mortality at 2 Wks	1.73%	1.71%	0.02%
Wt, kg	2.241	2.282	0.041
FCR	2.005	1.980	0.025 (1.25%)
Air Sac	0.61%	0.31%	0.3%
Total Condemns	2.20%	1.47%	0.73%

Case 6 — University Trial at Tennessee Tech

Used at a dilution of 1:2000 — May 15, 2023 – June 19, 2023

Summary of Both Rooms

Trt	Label	Day 35 Avg Bird Wt (lbs)	Day 0–35 Feed Intake (lbs)	Day 0–35 FCR (Not Mort. Corr.)	Day 0–35 FCR (Mort. Corr.)
1	Least Sq Mean	6.977^ab	9.138^a	1.329^a	1.347^ab
2	Least Sq Mean	6.930^b	9.120^a	1.334^a	1.354^a
3	Least Sq Mean	7.063^a	9.078^a	1.303^b	1.320^b
Δ TRT 1 vs 3		0.092	-0.059	-0.026	-0.027
% Change 1 vs 3		1.32%	-0.65%	-1.96%	-2.01%
N		96	96	96	96
Root Mean Square Error		0.096	0.163	0.038	0.060
Prob > F		0.0861	0.8156	0.0112	0.1047

TRT 1: Control · TRT 2: AQUATIZE (1:2000) Day 1–7 & Day 24–35 · TRT 3: AQUATIZE (1:2000) Day 24–35

Summary of Room 1 (West Wing)

Trt	Label	Day 35 Avg Bird Wt (lbs)	Day 0–35 Feed Intake (lbs)	Day 0–35 FCR (Not Mort. Corr.)	Day 0–35 FCR (Mort. Corr.)
1	Least Sq Mean	6.909^a	9.202^a	1.333^a	1.358^a
2	Least Sq Mean	7.098^a	8.974^ab	1.337^a	1.364^a
3	Least Sq Mean	7.196^a	8.713^b	1.271^a	1.260^a
Δ TRT 1 vs 3		0.286	-0.488	-0.062	-0.098
% Change 1 vs 3		4.14%	-5.31%	-4.64%	-7.23%
N		48	48	48	48
Root Mean Square Error		0.0950	0.1428	0.0291	0.0407
Prob > F		0.1641	0.0873	0.1091	0.0003

TRT 1: Control · TRT 2: AQUATIZE (1:2000) Day 1–7 & Day 24–35 · TRT 3: AQUATIZE (1:2000) Day 24–35

Case 7 — AQUATIZE Pen Trial at AHPharma

Salisbury, MD — November 2021

Trt	Description	Cocci Challenge	Treatment	Route	Reps	Birds/Rep
1	Challenged / No Treatment	Yes	None	N/A	12	52
2	AQUATIZE	Yes	AQUATIZE (1:2000) Day 1–21; (1:5000) Day 22–42	Drinking Water	12	52

• Corn and SBM rations, with normal nutritional formulations.
• AQUATIZE does no harm to live production personnel.
• No coccidiostat or ABF (Antibiotic Free Products) were administered during the entire study. One control antibiotic and four test materials were fed.
• Coccidiosis vaccine was administered at the hatchery or during the course of the study, with confirmation provided from the hatchery.

Days 1–42 Results

Metric	T1	T1 Sig.	T2	T2 Sig.	Difference
Average Body Wt (g)	2565.46	a	2634.89	b	69.43
Feed Conversion Corrected	1.809	a	1.703	b	-0.06
Mortality %	0.694	a	0.347	a	-0.35
Average Body Wt Gain (g)	2512.41	a	2582.15	a	69.74
Body Wt Coeff. of Variation	9.21	a	9.03	a	-0.18
Feed Consumption (g/bird/day)	111.02	a	110.48	a	-0.54
Avg g Gained/Day	61.08	a	62.74	a	1.66
European Production Efficiency	326.24	a	347.36	b	21.12

Significance at p < 0.05.

Case 8 — Field Trial at a USA Poultry Company

November 2023 — used at a dilution of 1:2000

Farm	Body Wt	Feed Conv.	Feed $/Ton	Birds Sold	AQUATIZE (gal)	$ AQ/Bird	$ Feed+AQ/Bird	g AQ/Bird
Greene	5.83	1.604	$350	71,654	100	$0.007	$1.653	3.364
Sarabella	5.63	1.630	$350	200,706	120	$0.012	$1.618	2.259
2 & K	5.48	1.626	$350	131,401	155	$0.014	$1.573	2.588
David Chase	5.57	1.608	$350	101,353	70	$0.014	$1.581	2.610
Izzy B	5.39	1.580	$350	139,779	90	$0.013	$1.503	2.433
Gary Stone #3	5.74	1.609	$350	102,652	50	$0.010	$1.626	1.840
A-Z	5.69	1.598	$350	210,896	90	$0.011	$1.606	2.808
HFC	6.09	1.579	$350	84,537	90	$0.021	$1.615	4.023
Total Birds Sold				1,033,888

Conversion & Feed Savings by Farm

Farm	Adj. Conv. Improvement	Net Conv. Impr. (pts)	Conv. Savings/Cost per Bird ($)	Net Feed Savings/Farm
Greene	-0.0414	-0.0250	-0.0255	$1,826.55
Sarabella	0.0181	0.0302	0.0298	-$5,979.20
2 & K	-0.0152	-0.0009	-0.0009	$115.41
David Chase	-0.0240	-0.0098	-0.0096	$971.05
Izzy B	-0.0205	-0.0068	-0.0065	$902.85
Gary Stone #3	-0.0518	-0.0421	-0.0423	$4,341.30
A-Z	-0.0926	-0.0777	-0.0773	$15,607.61
HFC	-0.0590	-0.0590	-0.0629	$5,315.62

Total Savings: $23,101.40

$0.0223 average savings per bird in trial

$22,344.20 feed savings / 1,000,000 birds / week

Case 9 — Salmonella in Field Trial at a USA Poultry Company

AQUATIZE used at various dilutions — summary

Average Salmonella (Control)	30,790
Salmonella (1/18) — AQUATIZE at 1:2000¹	11,719
Average Adjusted Feed Conversion Improvement²	2.64

¹ Of 18 houses analyzed, only one (1) house had Salmonella noted here.
² Average Feed Conversion Improvement in study using AQUATIZE at 1:2000.

Trial was discontinued due to lack of Salmonella in samples.

Case 10 — Bacterial Field Trial at a USA Poultry Company

AQUATIZE used at 1:2000 on TNTC houses — Arkansas Complex overview, fecal contamination

	TNTC	> 0 CFU	No Growth
Oct 2023 Water	107	45	41
December 2023 Water	50	18	175
March 2024 Water	3	12	221

AQUATIZE Water Program started in August 2023.

From the Field

Other Interesting Observations From Customers

✓Much drier feces.
✓More water and feed intake, especially in young animals.
✓No algae problems around drinking systems.
✓Facing an E. coli infection, a combination of antibiotics and AQUATIZE worked better than applying antibiotics alone.
✓Less total medication cost.